Specifications |
Appearance (Colour) |
Light tan |
Appearance (Form) |
Crystalline hygroscFree flowing, homogeneous powderopic compound |
Solubility |
65.00 gm/liter |
Solubility before autoclaving (Clarity) |
Clear to very slightly opalescent |
Gel strength (1.5% gel) |
Firm, comparable with 1.5% Agar gel |
pH (25°C) |
7.2 ± 0.2 |
Prepared Medium Appearance after autoclaving (Clarity) |
Opaque |
Prepared Medium Appearance after autoclaving (Colour) |
Yellow |
Cultural Response |
Inoculate and incubate at 37°C for 24- 48 hours |
Organism |
Staphylococcus aureus ATCC 25923 |
Inoculum (cfu) 10-100 |
Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+) |
Organism |
Staphylococcus aureus ATCC 6538 |
Inoculum (cfu) 10-100 |
Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+) |
Organism |
Proteus mirabilis ATCC 25933 |
Inoculum (cfu) 10-100 |
Growth : Good, Recovery Rate : >=50%, Colony colour : Brown – Black, Lecithinase halo : (-) |
Organism |
Staphylococcus epidermidis ATCC 12228 |
Inoculum (cfu) 10-100 |
Growth : Poor – good, Recovery Rate : 30 – 40%, Colony colour : Black, Lecithinase halo : (-) |
Organism |
Bacillus subtilis ATCC 6633 |
Inoculum (cfu) 10-100 |
Growth : Poor- fair, Recovery Rate : 0 – 10%, Colony colour : Brown -Black, Lecithinase halo : (-) |
Organism |
Escherichia coli ATCC 25922 |
Inoculum (cfu) 10-100 |
Growth : None – poor, Recovery Rate : 0 – 10%, Colony colour : Brown black, Lecithinase halo : (-) |
Organism |
Micrococcus luteus ATCC 10240 |
Inoculum (cfu) 10-100 |
Growth : None -poor, Recovery Rate : 30 – 40%, Colony colour : Very small shades of black -brown, Lecithinase halo : (-) |
Key |
Lecithinase halo : (+) indicates opaque zone around colony Lecithinase halo : (-) indicates no opaque zone around colony |
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