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Baird-Parker Agar, Base (B/S) – 100 Gms

1,000.00
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Barium Chloride Dihydrate ACS, 99% – 500 Gms

380.00

Baird-Parker Agar, Base (B/S) – 500 Gms

4,200.00

Application
Recommended medium for isolation and enumeration of coagulase positive staphylococci from food and other specimens.  
Composition
Ingredients gm/lt.
Agar 22
L-Glycine 12
Sodium pyruvate 10
Pancreatic digest of casein 10
Meat extract 5
Lithium chloride 5
Yeast extract 1

 

Directions
1. Add 65.00 gm powder to 940.0 ml distilled/purified water and mix thoroughly.
2. Gently heat and bring to boiling.
3. Autoclave at 15 psi pressure at 121°C for 15 minutes.
4. Cool to 45-50°C.
5. Add aseptically 10 ml sterile 1% Potassium tellurite solution (91488) and mix well.
6. Add 50 ml of sterile egg yolk emulsion.
7. Mix well.
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Description

Specifications
Appearance (Colour) Light tan
Appearance (Form) Crystalline hygroscFree flowing, homogeneous powderopic compound
Solubility 65.00 gm/liter
Solubility before autoclaving (Clarity) Clear to very slightly opalescent
Gel strength (1.5% gel) Firm, comparable with 1.5% Agar gel
pH (25°C) 7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Opaque
Prepared Medium Appearance after autoclaving (Colour) Yellow
Cultural Response Inoculate and incubate at 37°C for 24- 48 hours
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)
Organism Staphylococcus aureus ATCC 6538
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Grey -Black, Lecithinase halo : (+)
Organism Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100 Growth : Good, Recovery Rate : >=50%, Colony colour : Brown – Black, Lecithinase halo : (-)
Organism Staphylococcus epidermidis ATCC 12228
Inoculum (cfu) 10-100 Growth : Poor – good, Recovery Rate : 30 – 40%, Colony colour : Black, Lecithinase halo : (-)
Organism Bacillus subtilis ATCC 6633
Inoculum (cfu) 10-100 Growth : Poor- fair, Recovery Rate : 0 – 10%, Colony colour : Brown -Black, Lecithinase halo : (-)
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : None – poor, Recovery Rate : 0 – 10%, Colony colour : Brown black, Lecithinase halo : (-)
Organism Micrococcus luteus ATCC 10240
Inoculum (cfu) 10-100 Growth : None -poor, Recovery Rate : 30 – 40%, Colony colour : Very small shades of black -brown, Lecithinase halo : (-)
Key Lecithinase halo : (+) indicates opaque zone around colony Lecithinase halo : (-) indicates no opaque zone around colony

 

General Information
Storage 8 to 25°C (Cool & Dry Area)
Shelf Life 36 Months
IMDG Identification Not Regulated for Transport (Non-Haz)

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