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Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym(s) : Albumin bovine serum, Bovine albumin, BSA

CAS Number : 9048-46-8

EC Number : 232-936-2

MDL Number : MFCD00130384

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SKU: A7906 Category: Tags: , , ,



Quality Level 300
biological source bovine
assay ≥98%
form lyophilized powder
mol wt ~66 kDa
purified by heat shock fractionation
packaging poly bottle of
origin USA origin
technique(s) blood typing: suitable
microbiological culture: suitable
loss ≤5%
pH 7
solubility water: soluble (40 mg/ml)
UniProt accession no. P02769
storage temp. 2-8°C
Gene Information bovine … ALB(280717)



Bovine serum albumin has been used-

  • as a component of PCR (polymerase chain reaction) mix
  • in the preparation of dissociated cortical neurons
  • during immunofluorescent labeling
  • as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion


10, 50, 100, 500 g in poly bottle
1, 5, 10 kg in poly drum

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term “Fraction V” has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.


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