Description
PROPERTIES
| Quality Level | 100 |
| material | stainless steel column |
| agency | suitable for USP L43 |
| product line | Discovery® |
| feature | endcapped |
| manufacturer/tradename | Discovery® |
| packaging | 1 ea of |
| extent of labeling | 12% Carbon loading |
| technique(s) | HPLC: suitable LC/MS: suitable |
| L × I.D. | 15 cm × 4.6 mm |
| surface coverage | 15 cm × 4.6 mm |
| L × I.D. | 4 μmol/m2 |
| impurities | <10 ppm metals |
| matrix | silica gel, high purity, spherical particle platform fully porous particle |
| matrix active group | PFP (pentafluorophenyl) phase |
| particle size | 5 μm |
| pore size | 120 Å |
| surface area | 300 m2/g |
| operating pH range | 2 – 8 |
| application(s) | food and beverages |
| separation technique | reversed phase |
DESCRIPTION
General description
Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.
The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
Features and Benefits
- Unique selectivity
- Similar retention to C18 (sometimes requires stronger mobile phase)
- Excellent peak shape
- Stable, low-bleed LC-MS separations
- Scalable separations from 3 to 10μm particle sizes
Recommended products
Discover LiChropur reagents ideal for HPLC or LC-MS analysis
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
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