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EDTA Disodium Salt Dihydrate for molecular biology, 99.5% – 5 Kg

9,800.00
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EMB Agar – 500 Gms

3,500.00

EMB Agar – 100 Gms

800.00

Other Information
Applications
For the isolation, cultivation and differentiation of gram negative enteric bacteria based on lactose fermentation.
Composition
Ingredients gm/lt.
Peptone 10
Agar 13.5
Lactose 5
Sucrose 5
Dipotassium hydrogen phosphate 2
Eosin Y 0.4
Methylene Blue 0.065

 

Directions
1. Add 35.96 gm powder to distilled/purified water.
2. Bring volume to 1.0 liter and mix thoroughly.
3. Gently heat and bring to boiling.
4. Autoclave at 15 psi pressure at 121°C for 15 minutes.
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SKU: 95514 Category:

Description

SKU: 95514

 

Specifications
Appearance (Colour) Pinkish purple
Appearance (Form) Free flowing, homogeneous powder
Solubility 35.96 gm/liter
Solubility before autoclaving (Clarity) Opalescent, with a uniform precipitate
Gel strength (1.5% gel) Firm, comparable with 1.5% agar gel
pH (25°C) 7.2 ± 0.2
Prepared Medium Appearance after autoclaving (Clarity) Opalescent, with finely disperesed precipitate
Prepared Medium Appearance after autoclaving (Colour) Purple w/green orange cast
Cultural Response Inoculate and incubate at 37°C for 18 – 24 hours
Organism Escherichia coli ATCC 25922
Inoculum (cfu) 10-100 Growth : Good Recovery Rate : >= 50.0% Colony colour : Blue- black w/dark centers with green metallic sheen
Organism Enterobacter aerogenes ATCC 13048
Inoculum (cfu) 10-100 Growth : Good Recovery Rate : >= 50.0% Colony colour : Pink without sheen
Organism Salmonella typhimurium ATCC 14028
Inoculum (cfu) 10-100 Growth : Good Recovery Rate : >= 50.0% Colony colour : Colourless
Organism Klebsiella pneumoniae ATCC 13883
Inoculum (cfu) 10-100 Growth : Good Recovery Rate : >= 50.0% Colony colour : Pink mucoid
Organism Proteus mirabilis ATCC 25933
Inoculum (cfu) 10-100 Growth : Good Recovery Rate : >= 50.0% Colony colour : Colourless
Organism Enterococcus faecalis ATCC 29212
Inoculum (cfu) 10-100 Growth : Partially inhibited Colony colour : Colourless
Organism Staphylococcus aureus ATCC 25923
Inoculum (cfu) 10-100 Growth : Inhibited

 

General Information
Storage 8 to 25°C (Cool & Dry Area)
Shelf Life 36 Months
IMDG Identification Not Regulated for Transport (Non-Haz)

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