Description
PROPERTIES
| Quality Level | 200 |
| description | non-ionic |
| form | viscous liquid |
| mol wt | ~603 |
| CMC | 0.08 mM (20-25°C) |
| viscosity | 250-350 cP, neat(25 °C)(lit.) |
| density | 1.06 g/cm3 at 25 °C (lit.) |
| HLB | 13 |
| InChI | 1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3 |
| InChI key | JYCQQPHGFMYQCF-UHFFFAOYSA-N |
DESCRIPTION
General description
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.
Application
IGEPAL® CA-630 has been used:
- as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
- along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
- as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
- as a component of PI buffer along with NaH2PO4 to wash slides in FISH (fluorescence in situ hybridization) procedure
- for solubilizing and isolation of membrane proteins
Packaging
50, 100, 500 mL in poly bottle
Other Notes
Nonionic, non-denaturing detergent
Quality
Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.
Caution
May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.
Legal Information
IGEPAL is a registered trademark of Solvay
SAFETY INFORMATION
Pictograms : 
GHS05,GHS07,GHS09
Signal Word : Danger
Hazard Statements : H302 – H315 – H318 – H410
Precautionary Statements : P264 – P273 – P280 – P301 + P312 – P302 + P352 – P305 + P351 + P338
Hazard Classifications : Acute Tox. 4 Oral – Aquatic Acute 1 – Aquatic Chronic 1 – Eye Dam. 1 – Skin Irrit. 2
Storage Class Code : 10 – Combustible liquids
WGK : WGK 3
Flash Point(F) : 483.8 °F
Flash Point(C) : 251 °C
Personal Protective Equipment : dust mask type N95 (US), Eyeshields, Gloves
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