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Tetrahydrofuran 34865-2.5L

29,779.58

Triton™ X-100 T9284-100ML

11,288.70

T9284

Sigma-Aldrich

Triton? X-100

BioXtra

Synonym(s) : t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether

Linear Formula : t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10

CAS Number : 9036-19-5

Beilstein : 2315025

MDL number : MFCD00128254

PubChem Substance ID : 329826903

NACRES : NA.21

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SKU: T9284-100ML Category:

Description

PROPERTIES

Quality Level 200
description non-ionic
product line BioXtra
form liquid
mol wt micellar avg mol wt 80,000
average mol wt 625
aggregation number 100-155
technique(s) permeability assay: suitable
impurities ?0.05% Phosphorus (P)
ign. residue ?0.4%
CMC 0.2-0.9 mM (20-25?C)
transition temp cloud point 65 ?C
pour point ~7 ?C
solubility water: soluble
anion traces chloride (Cl-): ?0.05%
sulfate (SO42-): ?0.05%
cation traces Al: ?0.0005%
Ca: ?0.0005%
Cu: ?0.0005%
Fe: ?0.0005%
K: ?0.005%
Mg: ?0.0005%
NH4+: ?0.05%
Na: ?0.1%
Pb: ?0.001%
Zn: ?0.0005%
HLB 13.5
SMILES string CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key HNLXNOZHXNSSPN-UHFFFAOYSA-N

Description

General description

Triton? X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton? X-100 has been used:

  • Along with Span? 80 and Tween? 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
  • As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
  • In the blocking solution during immunocytochemistry procedure
  • During deglycosylation procedure
  • For membrane solubilization
  • For cell permeabilization
  • In decellularization of porcine heart valve tissue
Packaging

1 L in poly bottle

100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Span is a registered trademark of Croda International PLC

TWEEN is a registered trademark of Croda International PLC

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

SAFETY INFORMATION

Pictograms GHS05,GHS07,GHS09
Signal Word Danger
Hazard Statements H302 – H315 – H318 – H410
Precautionary Statements P264 – P273 – P280 – P301 + P312 – P302 + P352 – P305 + P351 + P338
Hazard Classifications Acute Tox. 4 Oral – Aquatic Acute 1 – Aquatic Chronic 1 – Eye Dam. 1 – Skin Irrit. 2
Storage Class Code 10 – Combustible liquids
WGK WGK 3
Flash Point(F) 483.8 ?F
Flash Point(C) 251 ?C
Personal Protective Equipment dust mask type N95 (US), Eyeshields, Gloves

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