Description
PROPERTIES
| Quality Level | 200 |
| description | non-ionic |
| product line | BioXtra |
| form | liquid |
| mol wt |
micellar avg mol wt 80,000 average mol wt 625 |
| aggregation number | 100-155 |
| technique(s) | permeability assay: suitable |
| impurities | ?0.05% Phosphorus (P) |
| ign. residue | ?0.4% |
| CMC | 0.2-0.9 mM (20-25?C) |
| transition temp |
cloud point 65 ?C pour point ~7 ?C |
| solubility | water: soluble |
| anion traces | chloride (Cl-): ?0.05% sulfate (SO42-): ?0.05% |
| cation traces | Al: ?0.0005% Ca: ?0.0005% Cu: ?0.0005% Fe: ?0.0005% K: ?0.005% Mg: ?0.0005% NH4+: ?0.05% Na: ?0.1% Pb: ?0.001% Zn: ?0.0005% |
| HLB | 13.5 |
| SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1 |
| InChI | 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3 |
| InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA-N |
Description
General description
Triton? X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Application
Triton? X-100 has been used:
- Along with Span? 80 and Tween? 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
- As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
- In the blocking solution during immunocytochemistry procedure
- During deglycosylation procedure
- For membrane solubilization
- For cell permeabilization
- In decellularization of porcine heart valve tissue
Packaging
1 L in poly bottle
100, 500 mL in poly bottle
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Legal Information
Span is a registered trademark of Croda International PLC
TWEEN is a registered trademark of Croda International PLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
SAFETY INFORMATION
| Pictograms | GHS05,GHS07,GHS09 |
| Signal Word | Danger |
| Hazard Statements | H302 – H315 – H318 – H410 |
| Precautionary Statements | P264 – P273 – P280 – P301 + P312 – P302 + P352 – P305 + P351 + P338 |
| Hazard Classifications | Acute Tox. 4 Oral – Aquatic Acute 1 – Aquatic Chronic 1 – Eye Dam. 1 – Skin Irrit. 2 |
| Storage Class Code | 10 – Combustible liquids |
| WGK | WGK 3 |
| Flash Point(F) | 483.8 ?F |
| Flash Point(C) | 251 ?C |
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
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