Description
PROPERTIES
| Quality Level | 200 |
| grade | laboratory grade |
| description | non-ionic |
| form | liquid |
| mol wt |
micellar avg mol wt 80,000 average mol wt 625 |
| aggregation number | 100-155 |
| CMC | 0.2-0.9 mM (20-25°C) |
| transition temp | cloud point 65 °C pour point ~7 °C |
| solubility | water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow |
| HLB | 13.5 |
| SMILES string | CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1 |
| InChI | 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3 |
| InChI key | HNLXNOZHXNSSPN-UHFFFAOYSA-N |
DESCRIPTION
General description
Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Triton X-100 has hemolytic property and is used for DNA extraction.
Application
Triton™-X has been used:
- In the permeabilization of cells for immunofluorescence staining
- As a component of lysis buffer in western blot analysis
- As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope
Packaging
5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle
Biochem/physiol Actions
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Legal Information
Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Reviews
There are no reviews yet.