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Triton™ X-100 – X100-5ML

2,497.50
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Triton™ X-100 – X100-100ML

5,372.40

Triton™ X-100 – X100-500ML

8,868.90

X100 Sigma-Aldrich

Triton X-100

laboratory grade

Synonym(s) : t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula : t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number : 9036-19-5
Beilstein : 2315025
MDL number : MFCD00128254
PubChem Substance ID : 329830772
NACRES : NA.21
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SKU: X100-500ML Category:

Description

PROPERTIES

Quality Level 200
grade laboratory grade
description non-ionic
form liquid
mol wt micellar avg mol wt 80,000
average mol wt 625
aggregation number 100-155
CMC 0.2-0.9 mM (20-25°C)
transition temp cloud point 65 °C
pour point ~7 °C
solubility water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow
HLB 13.5
SMILES string CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1
InChI 1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3
InChI key HNLXNOZHXNSSPN-UHFFFAOYSA-N

DESCRIPTION

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.
Triton X-100 has hemolytic property and is used for DNA extraction.

Application

Triton-X has been used:

  • In the permeabilization of cells for immunofluorescence staining
  • As a component of lysis buffer in western blot analysis
  • As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Packaging

5 mL in glass bottle
1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Tri-Chek is a trademark of Micro Essential Laboratory, Inc.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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