Previous

SP®-2560 Capillary GC Column

182,487.85
Next

Trizma® base T6066-500G

7,729.05

Trizma® base T6066-100G

6,462.53

T6066 Sigma-Aldrich

Trizma® base

BioPerformance Certified, meets EP, USP testing specifications, suitable for cell culture, ≥99.9% (titration)

Synonym(s) : Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris(hydroxymethyl)aminomethane
Linear Formula : NH2C(CH2OH)3
CAS Number : 77-86-1
Molecular Weight : 121.14
Beilstein : 741883
EC Number : 201-064-4
MDL number : MFCD00004679
PubChem Substance ID : 24900345
NACRES : NA.25
Add to Wishlist
Add to Wishlist
SKU: T6066-100G Category:

Description

PROPERTIES

 

grade BioPerformance Certified
Quality Level 400
agency USP/NF
meets EP testing specifications
meets USP testing specifications
description aminopeptidase substrate
assay ≥99.9% (titration)
form crystalline powder
technique(s) cell culture | mammalian: suitable
impurities DNase, RNase, protease, none detected
Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested
≤0.2% water (Karl Fischer)
loss ≤0.5% (loss on drying, 110 °C)
pH 10.0-11.5
useful pH range 7 – 9
pKa (25 °C) 8.1
bp 219-220 °C/10 mmHg (lit.)
mp 167-172 °C (lit.)
solubility water: soluble (666 mg/ml, yielding a clear colorless solution)
absorption ≤0.05 at 260 at 1 M
≤0.05 at 290 at 40%
≤0.06 at 280 at 1 M
application(s) diagnostic assay manufacturing
SMILES string NC(CO)(CO)CO
InChI 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key LENZDBCJOHFCAS-UHFFFAOYSA-N

DESCRIPTION

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Trizma has been used:

  • As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
  • As a component of pre-hybridization buffer in in situ hybridization
  • To suspend dried protein before SDS-PAGE protein characterization
  • As a component of lysis buffer to wash and lyse cells before immunoprecipitation
  • In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
  • In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
  • In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Packaging

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Reviews

There are no reviews yet.

Be the first to review “Trizma® base T6066-100G”

Your email address will not be published. Required fields are marked *

Shopping cart

0
image/svg+xml

No products in the cart.

Continue Shopping