Description
PROPERTIES
| grade | BioPerformance Certified |
| Quality Level | 400 |
| agency | USP/NF meets EP testing specifications meets USP testing specifications |
| description | aminopeptidase substrate |
| assay | ≥99.9% (titration) |
| form | crystalline powder |
| technique(s) | cell culture | mammalian: suitable |
| impurities | DNase, RNase, protease, none detected Endotoxin and Total Count Aerobic, Yeast, plus Mold, tested ≤0.2% water (Karl Fischer) |
| loss | ≤0.5% (loss on drying, 110 °C) |
| pH | 10.0-11.5 |
| useful pH range | 7 – 9 |
| pKa (25 °C) | 8.1 |
| bp | 219-220 °C/10 mmHg (lit.) |
| mp | 167-172 °C (lit.) |
| solubility | water: soluble (666 mg/ml, yielding a clear colorless solution) |
| absorption | ≤0.05 at 260 at 1 M ≤0.05 at 290 at 40% ≤0.06 at 280 at 1 M |
| application(s) | diagnostic assay manufacturing |
| SMILES string | NC(CO)(CO)CO |
| InChI | 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2 |
| InChI key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
DESCRIPTION
General description
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Application
Trizma has been used:
- As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
- As a component of pre-hybridization buffer in in situ hybridization
- To suspend dried protein before SDS-PAGE protein characterization
- As a component of lysis buffer to wash and lyse cells before immunoprecipitation
- In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
- In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
- In capillary electrochromatography and UV analysis of tocopherols and tocotrienols
Packaging
1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum
T6066-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
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